PrimerPickr
https://bio.tools/primerpickrPrimerpickr is an open-source tool for rt-PCr primer picking powered by the aggregation of public usage of rt-pcr primers from open source papers. The database is validated with 154 genes and contains over 31,000 genes across 10 species.
Sourced from
- bio.tools — primerpickr
Related resources
Estimates PCR primer melting temperatures and polymerase-specific annealing temperatures from sequence and buffer inputs, with per-pair QC for hairpins, dimers, and Tm balance. A browser calculator supports interactive single-pair and batch entry (up to 200 pairs) with method comparison and export; a Python library and command-line tool submit the same parameters to the Pepkio Tools API for scripted and pipeline use. Calculator arithmetic for the API client is hosted remotely; sequences are transmitted for programmatic runs while the web interface performs calculations in the browser.