BioMutation

BioMutation is a user-friendly graphical interface designed to streamline the process of biomolecular mutagenesis through an intuitive, step-by-step workflow. It iteratively utilizes the mutagenesis engines of UCSF ChimeraX, namely, swapna for nucleic acid point mutations and swapaa for amino acid point mutations, based on user-defined requirements. Built using modern web technologies (HTML, CSS, JavaScript), BioMutation is lightweight, runs locally on standard systems, and requires no installation beyond ChimeraX. With its user-friendly design and expanded capabilities, BioMutation can be a valuable GUI-based platform for high-throughput mutagenesis across biomolecular systems. The tool can be accessed as an online web server.