AnakinHuang/brainscope-scgpt-disease

# nano-scGPT The simplest, fastest repository for scGPT inference, (soon) finetuning and trianing, with minimal dependencies. It reimplements the original scGPT from scratch. nanoscgpt/model.py is pure PyTorch in ~270 lines of code, and nanoscgpt/scGPT_tokenizer.py turns raw scRNA data into model…

scRep is a PyTorch model for extracting cell embeddings from single-cell RNA-seq AnnData (.h5ad) inputs. This repository is a standalone Hugging Face release bundle: it includes checkpoint weights, the exact paired gene vocabulary, the inference implementation, and runnable examples.

GeneJEPA is a Joint-Embedding Predictive Architecture (JEPA) trained for self-supervised representation learning on scRNA-seq. It uses a Perceiver-style encoder to handle sparse, high-dimensional gene count vectors and a Fourier-feature tokenizer for numerical tokenization.

Automated cell type annotation tool for single-cell transcriptomics using gradient boosting and logistic regression with reference atlases, enabling standardized classification across datasets (Wellcome Sanger Institute, Nature Biotechnology 2022)

Single-cell mRNA sequencing can uncover novel cell-to-cell heterogeneity in gene expression levels in seemingly homogeneous populations of cells. However, these experiments are prone to high levels of technical noise, creating new challenges for identifying genes that show genuine heterogeneous expression within the population of cells under study. BASiCS (Bayesian Analysis of Single-Cell Sequencing data) is an integrated Bayesian hierarchical model to perform statistical analyses of single-cell RNA sequencing datasets in the context of supervised experiments (where the groups of cells of interest are known a priori, e.g. experimental conditions or cell types). BASiCS performs built-in data normalisation (global scaling) and technical noise quantification (based on spike-in genes). BASiCS provides an intuitive detection criterion for highly (or lowly) variable genes within a single group of cells. Additionally, BASiCS can compare gene expression patterns between two or more pre-specified groups of cells. Unlike traditional differential expression tools, BASiCS quantifies changes in expression that lie beyond comparisons of means, also allowing the study of changes in cell-to-cell heterogeneity. The latter can be quantified via a biological over-dispersion parameter that measures the excess of variability that is observed with respect to Poisson sampling noise, after normalisation and technical noise removal. Due to the strong mean/over-dispersion confounding that is typically observed for scRNA-seq datasets, BASiCS also tests for changes in residual over-dispersion, defined by residual values with respect to a global mean/over-dispersion trend.

Methods to infer clonal tree configuration for a population of cells using single-cell RNA-seq data (scRNA-seq), and possibly other data modalities. Methods are also provided to assign cells to inferred clones and explore differences in gene expression between clones. These methods can flexibly integrate information from imperfect clonal trees inferred based on bulk exome-seq data, and sparse variant alleles expressed in scRNA-seq data. A flexible beta-binomial error model that accounts for stochastic dropout events as well as systematic allelic imbalance is used.