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PeakBot is a python package for peak-picking in LC-HRMS profile mode data. It uses local-maxima in the LC-HRMS dataset each of which is then exported as a standarized two-dimensional area (rt x mz), which is used as the input for a machine-learning CNN model that reports whether the local-maxima is a chromatographic peak with left/right isomeric compounds or a signal of the background.